Figure 3
PGV-1 blocks progression of the M phase and induces apoptosis in vivo. (a) Experimental design to test the effects of PGV-1 in vivo. (b–e) MIA PaCa-2 cells were injected subcutaneously (1 × 107 cells) and into the pancreas (2 × 106 cells) of nude mice, and PBS (control) and PGV-1 (25 mg/kg BW) were orally administered every 2 days. On day 7, mice were sacrificed and tumors were resected. (b) Tumors were cut into small pieces in medium, and tumor cells were filtered, fixed onto glass slides by the cytospin technique, and stained with Hoechst 33342. Chromosomal DNA was viewed under confocal microscopy and DNA contents were measured. (c) Apoptosis indices were calculated at both the heterotopic and orthotopic sites and compared between the control and PGV-1 groups (Left panel; heterotopic site, Right panel; orthotopic site). (d) Microscopic image of the tumor after TUNEL staining in the PGV-1 group. (e) Comparison of microscopic images of tumors after TUNEL staining in CDX-M and CDOX between the control and PGV-1 groups (Left upper panel; CDX-M in the control group, Right upper panel; CDX-M in the PGV-1 group, Left lower panel; CDOX in the control group, Right lower panel; CDOX in the PGV-1 group). P values were calculated with Student’s t test. Data are the average of 2 independent experiments (b,c) shown as mean ± SEM.
