Figure 2

Cell proliferation is suppressed in Arf1-deficient BMMCs. (a) Either control (ctrl) or Arf1-KO BMMCs at 5-week after culture (n = 8, each) were stimulated with the indicated concentration of IL-3 for 5 days. The cell numbers were determined using Cell Counting Kit-8 and shown relative to the cell numbers at day 0 (mean ± SD). (b) Expression levels of CD123 and CD131 in BMMCs at 5-week after culture were evaluated by FACS (n = 3, each). Shown are representative FACS profiles (top) and mean fluorescent intensities (MFI) (bottom). Gray thin lines on FACS plot indicate negative stained signal with isotype controls. Mean ± SD. (c, d) The proportions of Ki67⁺ cells (c) and Annexin V⁺ cells (d) in BMMCs at 5-week after culture were evaluated by FACS (n = 4, each). Shown are representative FACS profiles (top) and proportions (bottom). Gray thin lines on FACS plot indicate negative stained signal with isotype controls (for Ki67) or control reagents (for Annexin V). Mean ± SD. **p < 0.01.