Table 3 Effect of supplementing Tris-extender with alpha-lipoic acid loaded liposomes (ALAN) on sperm characteristics (%) in post-thawed buffalo bull semen (at 37 °C for 30 s).

From: Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa

Treatment1

 

Sperm characteristics (%)2

Progressive motility

Vitality

Membrane Integrity

Acrosome Integrity

Abnormality

ALAN0

36.0 ± 1.87c

39.4 ± 2.75c

40.6 ± 2.40

90.6 ± 0.93

12.6 ± 0.93

ALAN25

40.0 ± 1.58bc

43.6 ± 1.94abc

43.0 ± 1.64

91.8 ± 0.86

11.6 ± 0.51

ALAN50

40.0 ± 1.58bc

42.2 ± 2.56bc

42.6 ± 3.19

92.6 ± 1.66

10.6 ± 0.68

ALAN75

45.0 ± 1.58ab

47.2 ± 1.59ab

48.0 ± 2.66

91.6 ± 1.03

10.6 ± 1.03

ALAN150

47.0 ± 2.00a

50.4 ± 2.56a

48.4 ± 2.38

91.0 ± 1.00

12.8 ± 0.97

  1. 1Tris extender supplemented with different levels of alpha-lipoic acid loaded liposomes (ALAN) containing 0 (ALAN0), 25 (ALAN25), 50 (ALAN50), 75 ALAN75 and 150 ALAN150 (0, 25, 50, 75 and 150 µg/mL), respectively.
  2. 2a–c means denoted within the same column with different superscripts are significantly different at P < 0.05.
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