Figure 3
Gelatin zymography for the investigation of gelatinolytic activity in unstimulated whole mouth saliva of 16 participants at four time-points. (a) Representative example of Coomassie-stained zymogram gel demonstrating bands with gelatinolytic activity at three different molecular weights (uncropped gel with original arrangement of lanes is presented in Supplementary Appendix Fig. S2). (b–d) Relative quantification of band intensity of bands 1, 2, and 3. The fold change of gelatinolytic activity at T2, T3, and T4 for each band was assessed relative to T1. T1 baseline (before placement of orthodontic appliance), T2 1 h after placement of orthodontic appliance, T3 1 week after placement of orthodontic appliance, T4 end of the alignment, M molecular weight markers, MMP-9 std matrix metalloproteinase-9 standard; *p < 0.05; **p < 0.01.
