Figure 5 | Scientific Reports

Figure 5

From: Improved β-cell function leads to improved glucose tolerance in a transgenic mouse expressing lipoprotein lipase in adipocytes

Figure 5

SREBP2 regulates Snd1 mRNA expression. (A,B) Differentiated 3T3-L1 adipocytes were treated with lipoprotein deficient serum containing media (LPDS) for 24-h. The cells were then incubated in LPDS or LPDS with cholesterol (Chol) as indicated. Real-time RT-PCR was used to measure the gene expression of Ldlr and Snd1. Next, differentiated 3T3-L1 adipocytes were treated with control (scrambled) or SREBP2 siRNA. (C) SREBP2 mRNA expression was measured. (D) SREBP2 (M:mature form) and actin were immunoblotted and quantified. (E) SND1 mRNA expression was determined. The data are represented as the mean ± SEM (n = 6 (A and B) or n = 3 (C–E); *P < 0.05; **P < 0.01; ***P < 0.001 two-tailed unpaired Student’s t-test).

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