Figure 2
From: Inhibition of inflammatory microglia by dietary fiber and short-chain fatty acids
Effect of dietary fiber intake on the TNF-α protein secretion (measured by ELISA) by primary microglia from mice fed 0 or 2.5% inulin for 2 months. TNF-α secretion by microglia was measured (A) after isolating and stimulating in vitro with LPS (0 or 1 ng/mL) or (B) after injecting mice i.p. with LPS (0 or 500 µg/kg) 4 h before isolating and culturing microglia 4 h, 8 h, 12 h, or 24 h. In (A), samples (0 or 2.5% inulin) were compared by unpaired t-test. Statistical difference between inulin levels was considered for P < 0.05. In (B), samples were analyzed by 2-way ANOVA (2 × 2 factorial arrangement of LPS and diet), followed by Šidák’s multiple comparisons test (*P < 0.05).
