Figure 5
From: Inhibition of inflammatory microglia by dietary fiber and short-chain fatty acids
Effect of short-chain fatty acids on nuclear translocation of p65 NF-κB in primary microglia from adult mice 30 min after stimulation with LPS. (Panel A) Fluorescence microscopy depicting immunocytochemical localization of NF-κB p65 (green) in primary microglia treated with no SCFA or SCFA mix (100 µM acetate + 100 µM butyrate) with no stimulation (two pictures on the left) and LPS 1 ng/mL stimulation (two pictures on the right). The white arrows point to the nucleus, indicating an example of the intensity of nuclear NF-κB in each treatment. (Panel B) Relative Nuclear p65 NF-kB of primary microglia, expressed as percentage of control (cells stimulated with LPS and no SCFA treatment). Primary microglia were plated and pre-treated with 0 or 100 µM acetate and butyrate for 4 h. After 30 min of LPS stimulation (0 or 1 ng/mL), cells were fixed and stained for p65 NF-κB. Values were expressed as the mean of four replicates, whose means resulted from 20 cells from three independent fields of view. Samples were analyzed by 2-way ANOVA (2 × 2 factorial arrangement of SCFA mix x LPS), followed by Šidák multiple comparisons test. Different lowercase letters are statistically different (P < 0.05) among treatments.
