Figure 6
From: Global lysine methylome profiling using systematically characterized affinity reagents
The landscape of the expanded lysine methylome. (a) Number of unique proteins and unique lysine methylation sites in the human lysine methylome and the percentage of sites identified in this study and those previously identified and recorded in the PhosphoSitePlus database. (b) Subcellular localization of all lysine methylated proteins compared to all human proteins (reviewed UniProt). (***) represents a p value < 2.0 e−16. (c) Over-representation analysis of all proteins with lysine methylation using the enrichGO function from the “clusterProfiler” package in R. Enriched ontologies—biological processes (“BP”), cellular compartments (“CC”), and molecular functions (“MF”)—identified using p. adjusted method of FDR with a cutoff of 0.05. (d) Analysis of the potential for PTM crosstalk. Percentage of Kme sites within 9 amino acids of another identified PTM (total sites, n = 10,413). All known acetylation, sumoylation, ubiquitination, and phosphorylation sites deposited from PhosphoSitePlus were used. Phosphorylation is subset by serine, threonine, and tyrosine sites.
