Figure 3 | Scientific Reports

Figure 3

From: Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors

Figure 3

Effects of SVF-EVs on lipogenic Differentiation of ADSCs in vitro. (A) Confocal microscopy image shows that ADSCs (green) endocytose PKH26-labeled SVF-EVs (red). (B) Images from Oil Red O staining for two groups (0 and 40 ng/mL proteins encapsulated in SVF-EVs) at different time points. (C) The quantitative statistics of Oil Red O positive area in two groups at different time points (*: p < 0.05, ***: p < 0.005, n = 5). (D) The qRT-PCR analysis of mRNA expression of lipogenic genes such as PPARγ, FAS, and ADPN in ADSCs (*: p < 0.05, n = 3). The western blot (E) and quantitative analysis (F) of the adipogenic-associative protein levels in ADSCs, reveals that the SVF-EVs promote lipogenic differentiation of ADSCs (*: p < 0.05, n = 3).

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