Table 4 Species discrimination success rate of Aquilaria, Dalbergia and Pterocarpus authentic wood sample using the neighbor-joining analysis based on single DNA regions and multi-loci combinations.
Genus | DNA barcode region and combination | Discrimination success rate | Number of monophyletic-species clade |
|---|---|---|---|
Aquilaria | ITS2 | 40% (2/5) | 3 |
matK | 40% (2/5) | 1 | |
trnL-trnF | 40% (2/5) | 1 | |
ITS2 + matK | 80% (4/5) | 3 | |
ITS2 + trnL-trnF | 100% (5/5) | 5 | |
matK + trnL-trnF | 80% (4/5) | 1 | |
ITS2 + matK + trnL-trnF | 100% (5/5) | 4 | |
Dalbergia | ITS2 | 100% (4/4) | 6 |
matK | 50% (2/4) | 7 | |
trnL | 50% (2/4) | 9 | |
trnH-psbA | 100% (4/4) | 10 | |
ITS2 + matK | 100% (4/4) | 12 | |
ITS2 + trnH-psbA | 100% (4/4) | 7 | |
ITS2 + trnL | 100% (4/4) | 5 | |
matK + trnH-psbA | 100% (4/4) | 9 | |
matK + trnL | 50% (2/4) | 7 | |
psbA-trnH + trnL | 100% (4/4) | 7 | |
ITS2 + matK + trnH-psbA | 100% (4/4) | 10 | |
ITS2 + matK + trnL | 100% (4/4) | 6 | |
ITS2 + psbA-trnH + trnL | 100% (4/4) | 6 | |
matK + psbA-trnH + trnL | 100% (4/4) | 6 | |
ITS2 + matK + trnH-psbA + trnL | 100% (4/4) | 5 | |
Pterocarpus | ITS2 | 66.67% (2/3) | 3 |
matK | 0% (0/3) | 0 | |
rbcL | 0% (0/3) | 2 | |
ndhF-rpl32 | 0% (0/3) | 0 | |
ITS2 + matK | 100% (3/3) | 1 | |
ITS2 + rbcL | 66.67% (2/3) | 2 | |
ITS2 + ndhF-rpl32 | 66.67% (2/3) | 1 | |
matK + rbcL | 66.67% (2/3) | 2 | |
matK + ndhF-rpl32 | 66.67% (2/3) | 1 | |
rbcL + ndhF-rpl32 | 66.67% (2/3) | 2 | |
ITS2 + matK + rbcL | 100% (3/3) | 2 | |
ITS2 + matK + ndhF-rpl32 | 100% (3/3) | 2 | |
ITS2 + rbcL + ndhF-rpl32 | 66.67% (2/3) | 2 | |
matK + rbcL + ndhF-rpl32 | 66.67% (2/3) | 2 | |
ITS2 + matK + rbcL + ndhF-rpl32 | 100% (3/3) | 2 |
