Figure 6
Investigations revealing the potential mechanism of Mage-D1 in mineralization. (a) Immunofluorescence double staining of Mage-D1, p75NTR, Dlx1 and Msx1 at E19.5 d rat dental germ. Scale bar represents 200 μm or 50 μm. (b) Co-immunoprecipitation shows that Mage-D1 can bind to p75NTR, Msx1, Dlx1 based on E19.5 d EMSCs. Full-length blots are presented in Supplementary Fig. S5. (c) Under induction with mineralized culture medium for 7 d, the expression levels of p75NTR, Msx1, Dlx1 were examined by real-time PCR normalized to GAPDH. For raw data see Supplementary Table 3. (**P < 0.01, ***P < 0.001). (d,e) Under induction with mineralized culture medium for 14 d, the expression levels of p75NTR, Msx1, Dlx1 were detected by Western blot analysis, GAPDH used as the reference gene. Full-length blots are presented in Supplementary Fig. S6.
