Figure 4
From: GSDMD deficiency ameliorates hyperoxia-induced BPD and ROP in neonatal mice
GSDMD-KO improves cell survival in hyperoxia-exposed lungs. (A) Immunofluorescent staining for Ki67 (pink signals, white arrows) and DAPI staining (blue signals) were performed to assess cell proliferation. (C) Quantification of the cell proliferation index (percentage of Ki67 positive nuclei divided by total nuclei) revealed that hyperoxia decreased cell proliferation in WT lungs but GSDMD-KO improved cell proliferation in hyperoxia-exposed lungs. (B) TUNEL Assay (green signals, white arrows) and DAPI nuclear stain (blue signals) were used to identify dead cells. (D) Quantification of cell death (percentage of apoptotic nuclei divided by total nuclei) revealed that WT lungs had increased cell death when exposed to hyperoxia. In contrast, hyperoxia-exposed KO lungs had significantly less cell death. n = 5/group. *P < 0.05, ***P < 0.001, WT-RA vs WT-O2. #P < 0.05, ###P < 0.001, WT-O2 vs KO-O2. 20 × magnification. Scale bars: 50 μm.
