Figure 2 | Scientific Reports

Figure 2

From: A high-throughput single-particle imaging platform for antibody characterization and a novel competition assay for therapeutic antibodies

Figure 2

Antibody screening for mAbs against EBOV GP on SP-IRIS using rVSV-EBOV model. (a) SP-IRIS signal (virus count/mm2) for 10 different mAbs as well as a negative antibody against wild-type VSV (8G5). Average virus densities were calculated from 3 replicate spots for each mAb after the SP-IRIS chip was incubated with 107 PFU/mL rVSV-EBOV for 1 h. (b) SP-IRIS signal drawn on a smaller density scale for the last 4 antibodies from (a) to show the signal levels clearly. The detection threshold is calculated from negative 8G5 antibody spots as average virus density plus three times the standard deviation. Any virus density above the detection threshold (2000 particles/mm2) is considered as a positive signal whereas antibodies with a lower signal than the threshold are considered to have no binding. Results given in Fig. 2 are representative of at least three independent experiments.

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