Figure 5
Specificity and sensitivity of 8.9F antibody for rVSV-LASV detection. (a) 8.9F antibody specificity test. Average virus densities on 8.9F spots (n = 6 spots) obtained from SP-IRIS images of two different chips incubated with either rVSV-LASV or rVSV-EBOV, both at a concentration of 5 × 104 PFU/mL. 8.9F antibody binds to rVSV-LASV whereas it does not show any significant binding for rVSV-EBOV chip. (b) Dilution series experiment for rVSV-LASV detection in FBS using 8.9F as the capture antibody (n = 6 spots). The lowest concentration detected is 105 PFU/mL, however, actual LOD is closer to 104 PFU/mL. Red dashed line represents the detection threshold obtained from 8.9F spots on a chip incubated with FBS alone and calculated as mean virus density plus three standard deviations. LOD experiment was performed at least three times in independent experiments.
