Figure 1

Time scale expression of Brachypodium distachyon central loop genes. Gene expression of two transcript variants of BdLHY [BdLHY1.1 (a) and BdLHY1.2 (b)] and BdTOC1 (c) was monitored by qRT-PCR over 76 h in total aerial parts (shoot) and total roots (root) of Brachypodium distachyon under two watering conditions [80% soil water content (80) and 40% soil water content (40)] and three lighting regimes (18:6 light:dark, 24:0 light and 0:24 dark). Plants were entrained for 4 weeks in 18:6 light: dark cycles (light period: 6:00 to 24:00; dark period: 24:00 to 6:00) before being exposed to the three different lighting regimes. As regards water status, plants were grown with 80% soil water content for 2 weeks before being subjected to modest water deprivation (40% soil water content) for 2 weeks before sampling. Bars represent the periods of night (grey bars) and subjective night (empty bars). Expression levels shown are relative to average expression of two reference genes (BdUBC18 and BdElFα) and relative to the mean expression level of the target gene over 76 h in shoots grown under 18:6 light:dark cycles and 80% soil water content. Data shown are from two representative experiments. The related period, phase, amplitude and relative amplitude error dataset can be found in Table 1. The standard deviation and related expression dataset from the two experiments are shown in Supplementary File 1.