Figure 3

Time scale expression of Brachypodium distachyon ELF-related genes. Gene expression of BdELF3 (a) and BdELF4-like genes [ELF4-like 3 (b), ELF4-like A (c) and ELF4-like B (d)] were monitored by qRT-PCR over 76 h in total aerial parts (shoot) and total roots (root) of Brachypodium distachyon under two watering conditions [80% soil water content (80) and 40% soil water content (40)] and three lighting regimes (18:6 light:dark, 24:0 light and 0:24 dark). Plants were entrained for 4 weeks in 18:6 light:dark cycles (light period: 6:00 to 24:00; dark period: 24:00 to 6:00) before being exposed to the three different lighting regimes. As regards water status, plants were grown under 80% soil water content for 2 weeks before being subjected to modest water deprivation (40% soil water content) for 2 weeks before sampling. Bars represent the periods of night (grey bars) and subjective night (empty bars). Expression levels shown are relative to the average expression of two reference genes (BdUBC18 and BdElFα) and relative to the mean expression level of the target gene over the 76 h in shoot grown under 18:6 light:dark cycles and 80% soil water content. Data shown are from two representative experiments. Related period, phase, amplitude and relative amplitude error dataset can be found in Supplementary Table S3. Standard deviation and related expression dataset from the two experiments are shown in Supplementary File 1.