Figure 4

Relative mean expression of Brachypodium GI (a), PRR95 (b), LUX (c) and ELF4-like B (d) genes in roots and shoots under three different lighting regime and two different kinds of soil water content. Mean transcript levels were calculated from overall relative transcript amounts of the target gene monitored by qRT-PCR every 4 h over a 76-h long time course experiment in total aerial parts (shoot) and total roots (root) of 4-week-old Brachypodium distachyon plants grown under two watering conditions [80% soil water content (80) and 40% soil water content (40)] and three lighting regimes [(18:6 light: dark (18:6 LD), 24:0 light (24:0 LL) and 0:24 dark (24:0 DD)]. Mean transcript levels were normalized to mean transcript level of shoots under 18:6 LD conditions and 80% soil water content. Plants were entrained for 4 weeks in 18:6 light: dark cycles before releasing them in the three different lighting regimes. As regards water status, plants were grown with 80% soil water content for 2 weeks before being subjected to modest water deprivation (40% soil water content) for 2 weeks before sampling. Error bars are ± SD for two independent experiments. Statistical analyses were performed by linear regression model and by linear regression supplemented with interacting prediction of the three different interactors: lighting regime, water status and plant part. Statistical testing of the models is summarized in Table 3. Values are statistically different (p < 0.05 by ANOVA) if interaction between the parameters is predicted. This figure presents genes with significant interaction. Relative mean expression values of BdLHY1.1, BdLHY1.2, TOC1, ELF3, ELF4-like 3 and ELF4-like A can be found in Supplementary Fig. S2.