Figure 1

Schematic representation of expression cassettes used for chloroplast transformation. (A) Partial sequence of uidA (GUS) gene fused in frame with psbC gene 5′ untranslated region (5′UTR). SD and SC represent Shine–Dalgarno sequence (blue) and start codon (yellow), respectively. The first six codons (following start codon) are highlighted in green. (B) Partial restriction map showing the chimeric genes uidA and aadA. Also shown is the site of wild-type tobacco plastome where the chimeric uidA and aadA genes are expected to be integrated into the chloroplast genome (crossed lines) and the resulting transplastome with restriction sites and anticipated size of DNA fragments (solid line with double arrow) when restricted with ClaI restriction enzyme. Nt., psbCP, psbCT, FLK(rbcL), FLK(aacD) represent Nicotiana tabacum, psbC promoter, psbC terminator, partial sequences from rbcL and accD genes as flanking sequences (FLK) on either side of the transgene cassettes, respectively.