Figure 2 | Scientific Reports

Figure 2

From: Chloroplasts evolved an additional layer of translational regulation based on non-AUG start codons for proteins with different turnover rates

Figure 2

Stable integration and expression of GUS in tobacco chloroplasts under the tobacco psbC promoter with four different start codons. Southern hybridization of total genomic DNA probed with uidA (A) and partial gene sequences of rbcL:accD (B). Lane 1ā€“6 correspond to size ladder (MW), wild type/controlĀ (Nt. Wt), psbC:AUG, psbC:CUG, psbC:TUG and psbC:GUG transplastomic Nicotiana tabacum (Nt.) plants, respectively. Note that in transcript/RNA Uracil (U) is used while in DNA/construct nucleotide Thymine (T) is used in place of Uracil. (C) Northern hybridization showing transcription of chimeric uidA under four different start codons in tobacco transplastomic plants. (D) The same blot reprobed with 16S rRNA to show equal loading of total RNA.

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