Figure 3 | Scientific Reports

Figure 3

From: Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle

Figure 3

AAV2/PHP.eB vector preference for the CA2 region in ex vivo organotypic slice cultures. (a) RFP, (b) GFP, and (c) RGS14 protein distribution in hippocampal slice cultures. AAV2/1-SynTetOff-mRFP1 and AAV2/PHP.eB-SynTetOff-EGFP were added to slice cultures at 0, 7, 14, and 28 DIV; RFP/GFP signals and RGS14 immunoreactivity were analyzed 48 h later. Scale bar, 200 μm. (d) Signal intensities of RFP (red), GFP (green), and RGS14 (gray) at each time point along the transverse axis of cultured hippocampal slices. Red, green, and gray areas indicate standard errors (n = 5 slices from 5 rats).

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