Figure 6 | Scientific Reports

Figure 6

From: Cell type specific IL-27p28 (IL-30) deletion in mice uncovers an unexpected regulatory function of IL-30 in autoimmune inflammation

Figure 6

IL-30 stimulation in CD4 T cell activation in vitro. (a) FACS sorted CD4+ naïve cells were stimulated with recombinant IL-27, IL-30. Phosphorylated STAT1 and STAT3 expression was determined by flow cytometry at 10- and 30-min following stimulation. (b,c) Naive CD4 T cells were stimulated under Th1 polarization conditions in the presence of IL-27 (filled bar) or IL-30 (open bar) (0–50 ng/mL) for 3 days. Ifng and Il10 mRNA expression was determined by qPCR. (d) Naïve CD4 T cells were incubated with media (Nil), 50 ng IL-27, or 50 ng IL-30 for one hour. The cells were then washed and restimulated with IL-27. Stat1 and Stat3 phosphorylation was determined by flow cytometry at 15 and 30 min following stimulation. The data shown are representative of two independent experiments.

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