Figure 2 | Scientific Reports

Figure 2

From: Aberrant promoter hypermethylation of miR-335 and miR-145 is involved in breast cancer PD-L1 overexpression

Figure 2

PD-L1 is a direct target of miR-335 and -145 in breast cancer cell lines. (a) MiR-335 and -145 downstream target genes predicted by miRDIP, target scan, and miRwalk (b) miR-335 and -145 are highly conserved across species and have binding sites within the 3′-UTR of human PD-L1 (c) MDA-MB231; BT549; and MCF7 cells were transfected with renilla and firefly luciferase expression constructs from psiCHEC KTM-2 harboring PD-L1 3′-UTR and miR-335 and -145, or scramble miR. Dual-Luciferase assays were done after 48 h. The relative Renilla luciferase activity went down, but the scramble miR had no effect. ns = non-significant, **P ≤ 0.01, ***P ≤ 0.001.

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