Figure 3

miR‐335 and -145 expressions were analyzed in BC tissue samples and cell lines. (a) Analysis of miR-335 and -145 expression in BC via TGCA samples (b)  The Kaplan–Meier plotter shows lower levels of miR-335 and -145 expression in primary breast tumors are associated with stronger and poorer, respectively, overall survival in the METABRIC (n = 1078) breast cancer patient datasets (c) miR‐335 and -145 expression were downregulated in BC tissues as compared with normal samples using qRT‐PCR (d) Receiver operating characteristics (ROC) and area under the curve (AUC) for miR-335 and -145 (e) Decreased expression of miR-335 and-145 was correlated with PD‐L1 mRNA level (r = − 0.6207, r = − 0.5431, respectively, Pearson correlation) (f) The correlation between miR-335 and-145 expression and the TNBC or non-TNBC status of the tumor (g) Expression of miR-335 and -145 in BC cell lines compared to normal breast tissue samples; ***P ≤ 0.001; **P ≤ 0.01; ns = non-significant. The results were expressed as the mean ± SE of the experiments. BC, Breast Cancer; mRNA, messenger RNA; PD‐L1, programmed death-ligand 1; qRT‐PCR, quantitative reverse transcription-PCR.