Figure 5
The effect of overexpression of miR-335 and -145 in the control of PD-L1 expression in mRNA and protein levels. (a) PD-L1 expression levels through miR‐335 and -145 transfection were analyzed using qRT‐PCR. The results showed that miR‐335 and -145 exogenous overexpression could significantly reduce PD-L1 expression at the mRNA level in BC cell lines. (b) Graphs represent PD‐L1 protein expression levels. (c) FACS analysis of PD‑L1 in breast cancer cell lines. Anti‑human PD‑L1 was used as a binding antibody to cell‑surface PD‑L1 protein. (d) Western blots showing changes in expression levels of PD-L1 protein after miRNA transfection in breast cancer cell lines and tissue. (e) Statistical histogram of the western blots (n = 3). GAPDH was used as a loading control. All data were presented as mean ± SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
