Figure 3
Differentially expressed genes and gene ontologies in the KIC5 mutant at 6 hpi, 24 hpi, and 36 hpi. Volcano plots showing differential gene expression at (a) 6 hpi [downregulated: 325 genes, upregulated: 317 genes], (b) 24 hpi [downregulated: 399 genes, upregulated: 397 genes], and (c) 36 hpi [downregulated: 279 genes, upregulated: 321 genes] (Supplementary Table S4). Compared to 12 hpi and 48 hpi, there are an increased number of DE genes at these three chosen timepoints. For each timepoint shown, R package pfGO (v 1.1) was used to perform gene ontology enrichment. Select upregulated and downregulated gene ontologies related to GO cellular compartment (CC), GO molecular function (MF), or GO biological process (BP) are displayed (Supplementary Table S9). Blue: downregulated genes, red: upregulated genes. Results plotted with log2-fold change (KIC5 mutant/NF54 WT) vs log2 p-value. FDR corrected p-value < 0.05; log2-fold change upregulated > 1.4, downregulated < − 1.4.
