Figure 4

Gut microbiota composition following three consecutive nights of adequate sleep (AS) or sleep restriction (SR). (a) Principal coordinates analysis of Bray–Curtis dissimilarities (PERMANOVA, P = 1.0). (b–d) Between-condition differences in α-diversity analysed by mixed model ANOVA. Mean ± SD shown. (e) Differential abundance analyses of phyla, genera and amplicon sequencing variants (ASVs) by Microbiome Multivariable Associations with Linear Models identified ASV_Firmicutes.Clostridia.Clostridiales.Ruminococcaceae as the only differentially abundant taxon (P ≤ 0.05; q ≤ 0.20). Median and interquartile range shown. n = 19.