Figure 4 | Scientific Reports

Figure 4

From: Expression of LIM domain-binding 3 (LDB3), a striated muscle Z-band alternatively spliced PDZ-motif protein in the nervous system

Figure 4

Immunolocalization of LDB3, filamin C and myotilin in spinal motor neurons. (A) Representative immunofluorescence on spinal cord lumbar cross section stained by GTX115593 antibody show LDB3 signal (green) in the cell body and processes of ChAT-positive (red) spinal motor neurons of six month old mice (n = 6). Addition of a blocking peptide results in loss of the LDB3 signal. (B, C) Immunostaining of parallel sections with antibodies against filamin C (B) and myotilin (C) detect both proteins in cholinergic neurons in the anterior horn of the spinal cord in mice (n = 6). Arrow heads indicate myotilin- and filamin C -antibody stained cells which are not ChAT positive. (D) Representative immunohostochemistry on human spinal cord lumbar segments (n = 3; 20 × magnification) show that neurons in the anterior horn react to the filamin C and myotilin antibody. Scale bar: 50 µm.

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