Table 1 uHPLC-MS/MS confirmation of enzymatic activity of brain and lung tissues of WT and KO rats. For rats, values shown are averages of duplicates from each of n = 6 rats per genotype ± standard deviation. For rabbit lung, values shown are averages of a duplicate ± standard deviation. TA = tryptamine, NF = peak not found.
Extracts | TA | NMT (nM) | DMT (nM) |
|---|---|---|---|
WT rat brain | − | 0.18 ± 0.09 | NF |
WT rat brain | + | 0.15 ± 0.09 | NF |
KO rat brain | − | 0.29 ± 0.13 | NF |
KO rat brain | + | 0.11 ± 0.06 | NF |
WT rat lung | − | 0.44 ± 0.22 | NF |
WT rat lung | + | 0.6 ± 0.67 | NF |
KO rat lung | − | 0.68 ± 0.23 | NF |
KO rat lung | + | 0.44 ± 0.26 | NF |
Rabbit lung | − | 0.08 ± 0.05 | NF |
Rabbit lung | + | 24.2 ± 13.2 | 0.57 ± 0.18 |
