Figure 2 | Scientific Reports

Figure 2

From: 3D osteogenic differentiation of human iPSCs reveals the role of TGFβ signal in the transition from progenitors to osteoblasts and osteoblasts to osteocytes

Figure 2

Dynamic behavior of hiPSCs during osteogenic induction. Horizontal (a) and vertical (b) snapshots of time-lapse imaging during the osteogenic induction of GFP-labeled hiPSCs (W2G) on type I collagen gel. Images were taken at the indicated times. Scale bars = 100 μm in (a) and X, Y: 508 μm and Z: 225 μm in (b). Confocal images of hiPSCs (404C2) in vertical (c) and horizontal (d) views during the osteogenic induction. Sections were stained with Phalloidin (green) and anti-COL 1 antibody (red). Arrowheads show cells surrounded by COL I.

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