Figure 3
(A–C) The relative number of EPP85-181RDB (A), EPP85-181RNOV (B), EPP85-181P (C) cells as the function of time measured after the administration of 50 nM paclitaxel alone and in combination with nsPEF. The study was conducted using CellcyteX automated cell counter and analyzer, ANOVA * p < 0.05, Black—Control, Red—PEF, Purple—Paclitaxel, Green—Paclitaxel added 24 h after PEF treatment; (D) Presto Blue assay after the exposure of the EPP85-181RDB, EPP85-181RNOV and EPP85-181P cells to 50 nM paclitaxel combined with nsPEF. The drug was added to the cells 24 h after nsPEF treatment and the cells were left for 48 h in the drug 50 nM solution, ANOVA *p < 0.05; (E) oscilloscope caption of nsPEF of 200 ns, 100 pulses, 10 kHz and 8 kV/cm; (F) computer modelling of the electrotherapy cuvette (1 mm gap), which shows an even distribution of the field inside the cuvette. Slight differences are observed in the periphery of the chamber.
