Figure 4
(A) Holotomographic microscopy photograph showing EPP85-181RDB cells 13 min after nsPEF treatment. White arrow indicates micro vesicles (MV’s) being detached from the cell membrane and yellow arrow shows already freely detached MV’s from the membrane; (B) the immunofluorescence staining of CD91 (green) and CD243 (red) 24 h following the exposure to nsPEF treatment of EPP85-181RDB cells; (C) colocalization studies between CD91 and CD243 signal shift 24 h after the cells were exposed to nsPEF, analyzed with LasX software; (D) signal distribution plots in the EPP85-181RDB cells exposed to nsPEF, the photographs were analyzed in ImageJ software via the distribution plot module; (E) proposed mechanism in which, the cells reduce the membrane content of MDR proteins via the release of MDR proteins containing vesicles.
