Figure 3

Size of myocardial infarction determined by Evan’s blue and TTC staining. (A) The result of Evan’s blue and TTC staining. The blue area is normal, and the remaining areas (including red and white) are ischemic risk zones (AAR), with white areas being myocardial infarction (IA) and red areas being ischemic but not infarcted. (B) Infarct size% was the ratio of IA to AAR. (C) AAR% was the ratio of AAR to the whole heart slide area. N = 5 for each group, group: y-AL youth fed ad libitum group, a-AL aged fed ad libitum group, a-CR aged caloric restriction group. One-way ANOVA was used to analyze the data. *P < 0.05 vs y-AL; #P < 0.05 vs a-AL.