Figure 5
From: Neuronal glutathione loss leads to neurodegeneration involving gasdermin activation
Synaptic pruning by C1q is involved in the reduced volume of GCLC-KO mouse brains. (A) Scheme showing PLX3397 (PLX) administration to mice for 2 months. GCLC-KO mice were fed a PLX3397 (PLX)-mixed diet or control diet for 2 months (from 3 to 5 months of age), and brain samples were collected at 5 months of age. (B–E) Brain sections from PLX- or control-chow treated GCLC-KO mice were immunostained with Iba1 antibody (B), stained with FluoroJade C (C), immunostained with GFAP antibody (D) or immunostained with C1q antibody (E). (B–D) Upper panels show the hippocampal CA1 region, and lower panels show the cortical region. (C) Magnified images of right panels show the hippocampal CA1 region (upper panels) and layers II–III of the cerebral cortex (lower panels) of PLX treated GCLC-KO mouse. Values shown in the graphs represent the mean intensity level ± SEM (n = 3; **p < 0.01). (E) Magnified images of lower panels show the hippocampal CA1 region (upper panels) and layers II-III of the cerebral cortex (lower panels). (F) C1q protein levels in the brains of PLX-treated or control mice were determined by western blotting. Values shown in the graph represent relative expression level of C1q (n = 3 (control) or 5 (PLX); ****p < 0.0001).
