Figure 3
From: Effects of imeglimin on mitochondrial function, AMPK activity, and gene expression in hepatocytes
LKB1-mediated phosphorylation of AMPK induced by imeglimin and metformin in HepG2 cells. (A) RT-qPCR analysis of LKB1 mRNA in cells transfected or not (control) with scrambled (siSCR) or LKB1 (siLKB1) siRNAs for 72 h. Data are from four independent experiments. (B, C) Immunoblot analysis of phosphorylated and total forms of AMPKα in cells transfected with LKB1-targeting or control siRNAs for 72 h and then stimulated with vehicle or with 3 or 10 mmol/l metformin or imeglimin for 3 h. Blots are shown on the left, and quantitative data for the pAMPKα/AMPKα ratio from three independent experiments on the right. Original blots are presented in Fig. S3. All quantitative data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns as determined either by one-way ANOVA followed by Tukey’s test (A) or by Student’s t test (B, C).
