Figure 4

Phosphorylation of AMPKα induced by imeglimin and metformin in the liver in vivo. (A, B) Blood glucose (A) and plasma insulin (B) concentrations at 1 h after i.p. injection of vehicle (control) or of metformin or imeglimin at 250 mg/kg in mice that had been maintained on a standard diet, deprived of food overnight, and allowed to refeed for 5 h. (C) Immunoblot analysis of phosphorylated and total forms of AMPKα in the liver of mice treated as in (A) and (B). Original blots are presented in Fig. S4. All quantitative data are means ± SEM (n = 4 mice). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns by Student’s t test (A, B) or one-way ANOVA followed by Tukey’s test (C).