Figure 7
In vivo activity tests of htM5. HFD-fed mice were injected with the wild type htFGF21 (htWT) or htM5 intraperitoneally at doses of 0.1 or 0.5 mg/kg once daily for 14 days. (A) Changes in body weight throughout the injection. (B) Percent weight gains on the following day after the last injection. (C) Effects of the injection on liver TG contents. (D) H&E and Oil Red O staining of liver sections showing better improvement in htM5 than htWT for the vacuole and lipid accumulation. Data are represented as mean ± SEM, calculated using two-way ANOVA followed by Tukey’s multiple comparisons test, *, #, and Ψp < 0.05, ** and ##p < 0.01, ***p < 0.001, ####p < 0.0001. n = 5–6 mice/group. *, **, and *** indicate vehicle-injected mice versus the htWT-injected mice. #, ##, and #### indicate vehicle-injected mice versus the htM5-injected mice. Ψ indicates the htWT-injected mice versus the htM5-injected mice. Scale bars: 100 μm (H&E), 50 μm (Oil Red O).
