Figure 1 | Scientific Reports

Figure 1

From: Allele-specific polymerase chain reaction can determine the diplotype of NUDT15 variants in patients with childhood acute lymphoblastic Leukemia

Figure 1

Primer designs for NUDT15 genotyping and allele-specific PCR. (A) Two primer sets, E1-F/E1-R and E3-F/E3-R, were used to amplify exons 1 and 3 of NUDT15, respectively. (B) Two sets of locked nucleic acid (LNA) primers were used to amplify samples with heterozygous NUDT15 c.55_56insGAGTCG and c.415C > T to differentiate between NUDT15*1/*2 and NUDT15*3/*6.

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