Figure 14
TH-IR axons innervated adipose tissue (white and brown fat cells) (a) Single optical section showed WAT morphology using a brightfield channel or (a’) fluorescent channel. b) Single optical section showed BAT morphology using brightfield or (b’) fluorescent channel. (c) Maximal intensity projection image showed both white and brown adipocytes near the atria being heavily innervated by TH-IR axons. (c’) Optical section of the same region in c indicating single TH-IR axons running through and around individual adipocytes. (c-e). Autofluorescent excitation by UV laser permitted clear visualization of the fat structure, with varicose axons around both WAT and BAT. Yellow autofluorescence, Green TH-IR.WAT white adipose tissue, BAT brown adipose tissue. Scale bar: 20 μm.
