Table 5 Representation of the significant difference related to the quantitative distribution of different immune response cells in organs of semi-heavy laying hens infected with Salmonella Typhimurium wild and mutant strains at different days post-infection.

Semi-heavy laying hens	1 DPI	3 DPI	7 DPI	14 DPI
Caecal tonsils
CD4⁺	Δ-STM**	ns	Δ-STM****	Δ-STM**
CD8⁺	ns	ns	ns	ns
Macrophages	Δ-STM**	ns	ns	Δ-STM****
Liver
CD4⁺	Δ-STM****	ns	Δ-STM*	Δ-STM**
CD8⁺	ns	ns	ns	Δ-STM***
Macrophages	Δ-STM***	ns	ns	Ns
Caecum
CD4⁺	ns	ns	ns	ns
CD8⁺	Δ-STM****	ns	ns	Δ-STM*
Macrophages	ns	ns	ns	Δ-STM*
Ileum
CD4⁺	Δ-STM**	ns	ns	ns
CD8⁺	ns	ns	ns	ns
Macrophages	ns	ns	ns	Δ-STM***

DPI, days post-infection; ∆-STM, Salmonella Typhimurium ∆ttrA∆pduA; wt-STM, Salmonella Typhimurium wild-type; ns, no significant difference. Within each organ and DPI, * means difference by two-way ANOVA followed by Bonferroni’s comparison test between wild and mutant strains values (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001). The strain presented in the table (∆-STM or wt-STM) as significant within the organ and DPI is that shown the major infiltration area to each cell.

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