Figure 1

Development of high-frequency Ca2+ waves in response to saponin treatment. Sequential X–Y images (top panels, taken every 250 ms) of the subepicardial regions in the left ventricular myocardium over a 1500-ms period as indicated by the horizontal lines below the electrocardiogram (ECG) under the control conditions (left) and 1 min after the introduction of the saponin treatment (right). The X-t images (lower panels) for the fluo4-fluorescence intensity of five different cardiomyocytes (cells 1–5) when evaluated along the c1–c5 lines (100 μm) identified in the top panels.