Figure 5
Immunostaining for estrogen- and progesterone-related factors in the control and Gp130 ecKO uteri. (a) Stromal ERα expression is increased in Gp130 ecKO uteri compared to the control at D4 (1600 h). PGR expression is comparable, but ALOX15 expression, downstream of PGR, is dramatically decreased in the uterine epithelium from Gp130 ecKO compared to the control. (b) Socs3 mRNA expression (dark purple in anti-sense probe) by ISH on D4 (1600 h). The sense probe shows a background signal. (c,d) The number of cells positive for F4/80, a marker of macrophages, is significantly increased in Gp130 ecKO stroma compared to the control at D4. Scale bars: (a) 100 µm (with lower magnification) and 50 µm (with higher magnification), (b) 100 µm, and (c) 50 µm. le luminal epithelium, ge glandular epithelium, st stroma.
