Figure 1 | Scientific Reports

Figure 1

From: White spot syndrome virus impact on the expression of immune genes and gut microbiome of black tiger shrimp Penaeus monodon

Figure 1

(A) PmSTAT and IE1 transcription levels in shrimp intestine at 24 h post-WSSV injection. PmSTAT was suppressed by injecting PmSTAT dsRNA 10 µg per shrimp’s gram, then 16 h later, followed by the same amount with the first injection. The control group was injected with PBS instead PmSTAT dsRNA. WSSV was injected at 24 h after the 2nd PmSTAT dsRNA injection. Shrimp intestines were collected at 24 h post-infection. In addition, we investigated the transcription levels of immune-related genes upon PmSTAT silencing during WSSV infection. (B) Transcription levels of interferon-like gene (PmVago 1, 4, and 5). (C) Expression levels of immune-related genes, including JAK/STAT pathway (PmDOME and PmJAK), Toll pathway (MyD88, Castus, Dorsal, and Spätzle), IMD pathway (Relish) and inhibitor of kappa B kinase (IKKβ, IKKε1, and IKKε2). Moreover, Transcription levels of immune genes were also observed. (D) Transcription levels of antimicrobial peptide and (E) phenol oxidase cascade.

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