Figure 4 | Scientific Reports

Figure 4

From: Whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniae

Figure 4The alternative text for this image may have been generated using AI.

Validation of MMA using defined monoclonal antibodies. (a) Schematic overview for the MMA. Bacteria labeled with SYTO 13 were printed to the nitrocellulose glass slide, and serotype specific monoclonal antibodies were then added to the microarray following Alexa Fluor 647 conjugated secondary antibody detection. (b) Printing layout of MMA. Isolated S. pneumoniae without a capsule was defined as serologically nontypeable (NT).89 Serotypes were shown and strain name is labeled in the parentheses. (c) Left panel of each figure indicate images of fluorescent signals observed on the microarray. Their corresponding targeted serotype of each antibody was labeled in red. All fluorescent intensities were normalized by the highest intensity value and shown as Normalized fluorescent intensity (NFI). Data shown correspond to the mean of triplicate spots and error bars represent means ± SD.

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