Figure 2

Alignment window of O. rufipogon accession CR100055 showing (a) a SNP at position 69/codon 23 in exon 1 of the CENH3 gene when compared to OsCENH3 reference. The SNP is highlighted in red circle. This variation is found in many other AA genome species accessions as well. (b) Non-synonymous substitution found in O. rufipogon accession CR100055 when aligned to reference at position 964/codon 61. Please also refer to Table 2. (The alignment was carried out using Geneious Prime version 2021.1.1; images were generated from Geneious Prime version 2021.1.1itself).