Figure 1
The densitometry of dopaminergic markers such as tyrosine hydroxylase (TH), dopamine transporter (DAT), dopamine D1-like receptor (DA1) and dopamine D2-like receptor (DA2) in the amygdala of ERβ knock-out (ERβ−/−) and wild-type (ERβ+/+) mice, n = 6 per group. Note that the volume density of TH (A), DAT (B) is significantly elevated in ERβ−/− mice when compared to ERβ+/+ mice whereas values for DA1 (C) do not differ in both mice lines but for DA2 (D) is significantly elevated in ERβ−/− mice. Additionally, note that the automated cells counting for DA1 (E) are not affected but DA2 (F) is significantly increased due to ERβ deficiency. Data are expressed as a box-and-whiskers plots, with the "box" depicting the median and the 25th and 75th quartiles, and "whiskers" showing 5th and 95th percentile. ** (p ≤ 0.01) and *** (p ≤ 0.001) indicates statistically significant differences between the studied groups of mice (ERβ−/− and ERβ+/+ mice) analysed by Student's t-test. Elements—immunoreactive somata, fibres and neuropil. LA—lateral nucleus, BL—basolateral nucleus, BM—basomedial nucleus, CO—cortical nucleus, ME—medial nucleus and CE—central nucleus.
