Figure 8
Representative colour photomicrographs illustrating the staining patterns of muscarinic acetylcholine type 1 receptor (AChRM1) and alpha-7 nicotinic acetylcholine receptor (AChRα7) in the amygdala basolateral nucleus (BL) of ERβ knock-out (ERβ−/−) and wild-type (ERβ+/+) mice, n = 6 per group. (A,B) ERβ−/− mice. (A′,B′) ERβ+/+ mice. Note increased density of AChRM1 and AChRα7 in ERβ−/− mice (A,B) when compared to ERβ+/+ littermates (A′,B′). Note also strong neuropil staining in AChRM1 preparations (A,A′) which makes it difficult to discern cells (arrows) from the background. The scale bar applies to all microphotographs, and it corresponds to the length of 50 µm.
