Figure 5
KU60019 Combined with VP-16 suppressed the survival of lung cancer cells. (A, C) Cells were treated with the indicated concentrations of KU60019 for 72 h, followed by the ATP-lite assay. (B, D) Cells were treated with various concentrations of VP-16 or in conjunction with an IC20 concentration of KU60019 and subjected to the ATP-lite assay. (E–H) H1299 and A549 cells were plated in triplicate in 60-mm dishes and treated with VP-16 (0.5 μM) alone or in conjunction with KU60019 (1 μM). After 10–14 days, colonies were stained and counted (> 50 cells in a colony). All experiments were independently repeated three times.
