Figure 3
Relative enrichment of 15N-nitrate in tissues after dietary intervention analyzed by LC–MS/MS. (A) 15N-labeled nitrate content was measured by LC–MS/MS. All nitrate ions in samples were first reduced to nitrite by bacterial nitrate reductases (Aspergillus niger). Then nitrite derivatization by DAN was performed to yield NAT. The result was based on the percentage ratio of 15NAT/(14NAT + 15NAT). Data are plotted as average ± standard deviation (n = 4–6, * p < 0.05). (B) The proportion of 15N-labeled nitrate in control and 3-day Na15NO3 supplementation group is shown.
