Figure 9

CLSM images of the fixed A549 cells (PFA) that were previously incubated with PSNP-NR-3 (a–c, particle concentration 100 µg/mL), SiO₂-RhB-3 (d–f, particle concentration 100 µg/mL), and 3 (g–I, dye concentration 35 µg/mL) for 30 min at a pH 4.5 in the presence of the H⁺/K⁺ ionophore nigericin. The nuclei were stained with DAPI. Transmitted light (a,d,g); merged blue (DAPI) and green (pH probe 3) channels: (b,e,h); and merged blue (DAPI), green (pH probe 3) and red (reference dyes, NR or RhB) channels: (c,f,i). The following measurements conditions were used: λ_Ex = 405 nm, λ_Em1 = 420-480 nm and λ_Em2 = 520–560 nm for DAPI and 3, and λ_Ex = 560 nm and λ_Em = 570-660 nm for the red reference dyes. All images show a scale bar of 10 µm.