Figure 3
From: PRMT5 is a therapeutic target in choroidal neovascularization
PRMT5 overexpression promotes cell growth. (a) Immunoblot, showing FLAG-tagged wild-type (wt) PRMT5 successfully overexpressed (left panel) or knocked down (right panel) in HRECs. (b) Immunoblot, showing FLAG-tagged wtPRMT5 successfully overexpressed (left panel) or knocked down (right panel) in iCEC2 cells. See also Supplementary Fig. S3. (c–f) Effect of PRMT5 on cell proliferation. Overexpression of wtPRMT5 promoted cell growth in HRECs (c) and iCEC2 cells (d), while shPRMT5 knockdown had the opposite effect in HRECs (e) and iCEC2 cells (f). Mean ± SEM, n = 3–4 biological replicates. *p < 0.05 wtPRMT5 vs. pLV-empty; #p < 0.05, shPRMT5 vs. shScramble, unpaired Student’s t-tests with Welch’s correction.
